Saima‘s paper funded by the ERC characterising the collagens in the developing skeletal rudiment was published in eCM. We used immuno-fluorescence to look at the major and minor collagens in sections of the humerus at TS22 (e13.5, before formation of the primary ossification centre), TS25 (e15.5) and TS27 (e17.5).
Several collagens change substantially with the progression of the ossification centre, like for example Col V.
Some develop amazingly complex structures over the period of development studied, like Col II (green) and XI (grey).
Congratulations Saima on this beautiful work!
Developmental Biomechanics 